ABSTRACT
Background: Colorectal carcinogenesis has been found to be associated with the polymorphic status of Toll-like receptor 4 gene in various populations of the world
Aim: The aim of the study was to determine the genetic association of TLR4 gene polymorphisms [Asp299Gly and Thr399Ile] with disease susceptibility and risk development in colorectal cancer [CRC] patients of Kashmir, India
Materials and methods: Genotype frequencies of TLR4 polymorphisms [Asp299Gly and Thr399Ile] were compared between 120 CRC patients and 200 healthy controls using PCRRFLP method
Results: We did not find any significant association between the TLR4 gene polymorphisms and the CRC cases [p>0.05]. However CT genotype [Thr399Ile] showed modest elevated risk of the development of CRC [OR = 1.78 95% CI [0.88-3.5]]. Also G allele [AG genotype] of TLR-4 Asp299Gly polymorphism was found to be significantly associated with the male gender [p value = 0.006] and involvement of Nodes [p value = 0.01] whereas, T allele [CT genotype] of Thr399Ile polymorphism showed significant association with the smoking status [p value = 0.03]
Conclusion: Our results suggest that TLR4 gene polymorphism is not a key modulator of the risk of developing colorectal cancer in Kashmiri population